How to choose suitable solvent model for molecular dynamics?

I had ran molecular dynamic simulation on apo protein using TIP3P, TIP4P, TIP4PEW and SPC for 25 ns to establish the most suitable solvent model for specific protein. However, none of them gave...

04 May 2018 7,759 3 View

What is the number of cells and which culture plate that need to used for growth curve assay?

How much the cells that I need to use for developing growth curve of the cells? What is the size of cell culture plate that I need to use? Is there any standard guideline to follow? At the moment,...

06 July 2016 4,362 4 View

What the software can be use to predict the side effect of the compound?

03 April 2016 1,531 4 View

What is the different between conformation generated using LigPrep and ConfGen?

In library preparation, LigPrep will be used followed by ConfGen. Both of software will generate conformation. So, is it necessary to do ConfGen after LigPrep because both will generate the...

08 September 2015 1,767 0 View

Do I need to do ligand preparation and generate conformation before converting into ldb format?

I want to screen the compounds in Ligand Scout. So I need to convert the compound to lbd format as Ligand Scout only accept that format. Is it I need to do ligand preparation and generate...

07 August 2015 5,999 3 View

What method can be used for developing multi-target inhibitor using in silico technique?

Reliable method that can be used.

02 March 2015 4,218 2 View

Is it necessary to measure the cytokines level as well as their protein expression at the same time?

May I know the different if we measure the cytokine level using ELISA and protein expression (Western blot and RT-PCR)? Some of the journal measure both.

01 February 2015 3,966 3 View

How to download database from .sh type files?

I have download the chemical database in .sh type files. How can extract it to .sdf files? I'm using Windows.

11 December 2014 9,852 2 View

What is the best animal model for rheumatoid arthritis?

What the different between all the model? Preferably rat/mouse.

09 October 2014 3,292 9 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

Is anyone familiar with the cyclic compressive fatigue testing (BS EN 12697 - 25) ?

I would like to get more information on the cyclic compressive fatigue testing (BS EN 12697 - 25) which is typically done for bituminous material in pavement design. Any one can advise on the...

28 February 2021 2,004 3 View

Can we recognize signs of the "volatility clustering" property of a time series in its time domain plot?

My purpose is to recognize the volatility clustering property without statistical tests such as The Lagrange Multiplier (LM) test.

24 February 2021 4,150 3 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

How we can measure Kemp % in wool without using of benzene?

Would you please let me to know about the methods for measurement of medullation and Kemp % on animal? My problem is that the wool sampling and sending to laboratory is not possible. So I would...

21 February 2021 7,260 4 View

Which statistical test to use for comparison of different groups in a categorization?

I am doing a study on how programmers with different levels of expertise choose class names. A description was provided to the participants together with a list of possible class names for the...

21 February 2021 3,662 4 View

Statistical Tests for Quantitative Study?

Hello Everyone, I have one population, sample size 200. and needed to find the correlation between Variable A and B. Also, which T-test should I choose for hypothesis testing Please advise...

21 February 2021 7,591 4 View

Methodological confusions regarding deductive and inductive reasoning, hypothesis and theory test?

Hi Colleagues- What do you think about the following methodological issues: 1. Is it methodologically wrong to formulate quantitative research questionnaire based on inductive reasoning? 2. Is it...

21 February 2021 4,634 6 View