How can the interference of excipients in the dissolution results be minimized when the quantitative procedure is by UV-vis spectrophotometry?
The use of UV-vis spectrophotometer without purification is not successful to any samples containing many interfering compounds.
Further, direct application of UV-vis spectrophotometer without purification does not obey the Lambert-Beer law.
Therefore, HPLC-photometric and/or HPLC-fluorometric method is strongly recommended (please see file; Insulin RP-HPLC).
Dear Vipul,
This is one major problem I encountered as a Quality Control Analyst in the pharmaceutical industry. In such case one ends up taking readings of garbage particulate matter. I remembered I had requested for the procurement of HPLC although very expensive but was granted because of the shortcomings of the UV-VIS spectrophotometer in this case
Excipient such as coloring agents e.g titanium oxide, binding agents etc are not only problematic to dissolution tests but to the entire assay of the finished product.
Aside excipients, drugs with more than two Active Pharmaceutical Ingredients (API) do also interfere with each other. This is why analytical researchers are contantly in search and in modification of assay methods, hence the updates of the pharmaceutical reference guides.
Although some various extraction techniques, the use of charcoal etc somehow have created some forms of relief.
I applaud your project and hope you are making substantial progress. I wish you the best.
Regards.
You can try 1st or 2nd derivative analysis though I am not hopeful. Your dissolution medium may also contain Citric Acid to complex and precipitate any potential metallic placebo interferences.
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