How can the amount of differences between two ∆G in MFOLD software might be significant?

03 March 2015 1 3K Report

I want to calculate ∆G and compare the stability of two secondary structures of two sequences, One in the Wild type and another in mutated forms that I found through the linkage analysis and confirming by sequencing , using MFOLD online software. I don't know how amount of differences between two ∆G in MFOLD software might be significant. Moreover, how many nucleotides around I have to select to achieving reliable results?

Reza Mozafari

Thank you Andrey! despite I read your valuable links but I couldn't find the data that I am looking for, I have result that it shows difference between two ∆G from inserted sequences less than a unit, so now I don't know how I can interpret it as significant relationship. In addition, I inserted 500 bp around the variation found during analysis that I'm not sure that it might be enough and the results are reliable or not. ?

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