Reza Mozafari

5 Questions 19 Answers 0 Followers

Questions related from Reza Mozafari

What is the PCR based approach to genotype up to a hundred SNPs in human?

Hello! I am looking for an accurate customizable technique to genotype up to a hundred SNPs, a range from 50 to 100. The SNPs are known but are located in different genes. The experiment assumed...

08 August 2019 7,303 7 View

How to generate a short sequence that does NOT align to the hg38?

I would like to find a short sequence (25 bp) that does not match anywhere within Human Reference Genome (hg38/hg19) through R scripts. I am not sure if there is a quick solution to do what I...

01 January 2019 3,708 3 View

Sequence alignment algorithm for big size genes in Matlab

When I employ the "Sequence Alignment application" of Matlab on big size genes, I stop by an error regards to the matrix array size. That's why the requested array exceeds maximum array size...

05 May 2017 2,123 6 View

How I can explain the difference in gene size in hg19 and NG genome coordinates?

I was looking at the gene coordinates of LDLR for the hg19 assenbly and NG RefSeq in order to convert them to each other, while I do understand the length of the gene may be different in the two...

03 March 2017 2,690 2 View

How can the amount of differences between two ∆G in MFOLD software might be significant?

I want to calculate ∆G and compare the stability of two secondary structures of two sequences, One in the Wild type and another in mutated forms that I found through the linkage analysis and...

03 March 2015 3,253 1 View