I am working on radiolabeling of fatty acid nanoparticles for a couple of months and facing the issue of not achieving the radiolabeling more than 50% whatever conditions I change to optimize including precursor's concentration, reducing agent's concentration, radioactivity concentration, pH, stirring and incubation time. My nano-formulations are temperature sensitive. Even syringe filtres couldn't filter the radiocolloid to the extent we require. I am open to any suggestions to solve this issue to reach 90% radiolabeling or separate out the radiolabeled nanoparticles from the whole solution.
Hi
A tough situation indeed. But can you please tell me how you identified the presence of reduced technetium in the preparation in presence of radiolabeled nanoparticles? I assume both radiolabeled nanoparticles and reduced technetium will remain at the point of spotting (if you are using TLC). As indicated by Andras Polyak, SEC could separate the three technetium species and, to some extent this technique can be used to obtain pure techentium fatty acid nanoparticle fraction for further experiment. Bulk purification may be difficult by this method.
To the best of knowledge and experience on separation science, employ a supported liquid membrane /with suitable nafion membrane, which willl keep separating the two species based on mass/structure there by leading to concentration of the lalbelled FA in one compartment , Needn't worry about 50% labelling. Labelled products keep getting isolated dynAmically by membrane separation.Expert opinion. Can be still sought to choose right membrane of high throughput.
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