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More Ihssan Samara's questions See All
I would like to know if anyone has experienced problems in the transformation of pichia pastoris GS115, using vector pPIC9?

I am trying to transform pichia pastoris GS115, but when I run the negative control (the cells without the DNA) it is also growing in the selection medium Minimal Dextrose Medium:1.34% YNB 4 ×...

04 July 2023 920 6 View

How to calculate the interactive effect (additive, antagonistic and synergistic) between stressors on biochemical parameters?

I am evaluating the isolated and combined effects of two stressors on physiological and biochemical parameters of fish. I analyzed different markers (eg antioxidant enzymes, DNA damage,...

17 April 2023 7,549 2 View

PVDF vs PES, which membrane is better at retaining extracellular vesicles during filtersterilization of biofluids before and after isolation process?

PVDF vs PES, which membrane is better at retaining extracellular vesicles during filter sterilization of biofluids before and after the isolation process?

18 January 2023 313 0 View

Where can I find Datasets for Reflection-based DDoS attacks on VANET?

I need a dataset related to Vehicular ad hoc Networks for Reflection-based DDoS attacks. Any idea or suggestion is welcomed. Thanks you!

24 June 2022 6,492 3 View

What should be the problem so that protein transfer does not occur in western blotting?

Hi, we are having a problem with protein transfer. We used the PVDF membrane (0.45 um) activated by methanol (20s). The pre-stained marker and a recombinant protein that we used as a control were...

23 March 2022 8,644 3 View

What s the epistemological position and reasoning adopted in the article : Crisis as Change Strategy in Public Organizations ?

Hello, i'm a novice researcher and i m having a problem analyzing an article i do understand the methodology used and results but i can't figure out the epistemological position or the reasoning...

22 July 2021 744 2 View

How to obtain 3d structure of protein?

I want to download 3d structures of two proteins of Acinetobacter Baumannii but am finding difficulty on PDB. can anyone guide me how to view or download 3d structures of my proteins (1....

02 December 2020 4,678 4 View

Why do I have multiple peaks in my melt curve?

In the primer validation, with the serial dilution of the cDNA, I have a melt curve with a single peak. However, when I do the gene expression using control and treatment samples (I am evaluating...

17 August 2020 3,028 5 View

When I can find 3D printer for Composite materials?

If I find this type of printer, can it print very precision pieces?

17 February 2020 2,854 2 View

Control of UPFC?

I'm working on Control Systems of advanced UPFC. For continuing research I need an help me about this

11 April 2019 4,501 2 View

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