Dear Dr. Jawad K.

Quantitative estimation of crude oils degraded by fungi can be done by subtracting the remaining oil after the incubation period from the initiat oil concentration used at time zero.

Best regard

Dear Dr. Mohammad

Many thanks for answering the question, but are sure there are no other interacting factors that can affect the accuracy of the results?

Best regards

Dear Jawad,

The accuracy of your results will depend on the method or methods used to determine the oil content. Simple, cheap and fast methods are usually more sensitive for interferences resulting in strange or incorrect results than more elaborate complicated and time consuming methods. Do spent time in validating methods before applying them.

Dear C. A. (Kees) Kan

Thanks a lot for the kind response. Yes, certainly need to look for a more accurate procedure to avoid time-consuming. Best regards

Quantitative estimation of crude oil degradation using gravimetry The percentage degradation of the crude oil was then calculated as:

Weight of crude oil(initial) −weight of crude oil (after treatment) / Weight of crude oil(initial ) ×100.

Or Qualitative analysis of crude oil degradation using gas chromatography

this answer according to my information

with my best wishes

Dear Dr. Neeran

Thanks for the valuable information, which is quite useful. Best regards

Dear Dr.Hero

My gratitude for the important comments, which I have to follow. My regards.

By using HPLC

The basic answer to your question is given by @ Mohammed Fayyadh above. The choice of method for the quantitative determination of crude oil is upon you. Everything depends upon the facilities of your laboratory.

Best regards

Vit

I want to underline that oil is not a simple well defined chemical entity, but rather a (very) complex mixture of many different molecules. It is (almost) impossible to identify all the compounds in an oil mixture, so any method used will be an approximation with certain assumptions (implicitly or explicitly) made. The composition of the oils and the method used will thus determine whether your answers are valid or not.

Dear Vit

Thank for comments, I fact, I would prefer the suggestions described by Kees that all the compounds in oil mixture should be identified together with the method conducted. Best regards.

Jawad

Dear Dr. Magda

Thanks, Dr. for the comment. Best regards

Jawad

Dear Dr. C. A. (Kees) Kan

Many thanks for valuable suggestions, it is really important to Identify the compositions of the oil mixture and to determine quantitatively and qualitatively of crude oil after the degradation process by fungi. But I would very grateful indeed if you kindly have any relevant references. Best wishes

Jawad

Being retired for many years, I do not know recent relevant papers. My comments originate from experience on problems we had many years ago with fat analysis, in which unexpected compounds heavily influenced results and conclusions.

Dear C.A. (Kees) Kan

Thanks for the valuable advice. Good luck.

Jawad