We need to explain the adaptative response and the phenotypical change of yeast by the production of proteins at increased temperature. How proteomic method can be used to notify the change of proteins.
Dear all, I'm wondering about a possible substitution of a G>A base or a deletion of the G base in view of the chromatograms I've analyzed. I would like to point out that I have observed the...
21 November 2023 8,904 2 View
Dear all, as part of my Master thesis, I am conducting a two-way repeated measures ANOVA on data from a social evaluation paradigm. The purpose for running this analysis is to investigate whether...
19 October 2023 3,746 1 View
Dear Sir/Madam, I am looking for a deletion or insertion anomaly in the CALR gene. After sequencing the same sample (Forward/Reverse), here's what I get. From the chromatograms we deduced that...
09 August 2023 4,907 4 View
I recently noticed that the BSC-DREAM8b-Dust model can no longer be accessed. http://www.bsc.es/ESS/bsc-dust-daily-forecast Do you have any idea if it can be accessed from another database or if...
11 March 2023 2,604 3 View
I want to culture and store fungal spores of aspergillus using glycerol and need help with the procedure from anyone using it or have used it.
14 September 2022 9,087 3 View
Hello scientists. I am seeking for natural compounds with anticancer activities. Actually I am interested to hepatocellular carcinoma (HCC). Unfortunately I am facing some issues to induce HCC...
09 June 2022 5,430 0 View
Hello my Dear, I have a question about CALR chromatogram analysis. I sequenced and aligned the amplified sequences with the reference sequences but I'm having trouble identifying the anomalies...
13 April 2022 756 8 View
Hello, recently we have been working with the cyanobacterium Arthrospira platensis (Spirulina) and noticed, in shake flask culture cultured on an orbital shaker at 100 rpm, spherical cells and no...
01 February 2022 6,501 5 View
Greetings, we are currently working on the cultivation of the microalgae Dunaliella tertiolecta. We try to count the cells in a Thoma cell counting chamber under the microscrope. Problem is, the...
10 August 2021 7,640 4 View
Currently we are working on the cultivation of Aspergillus oryzae in shake flask liquid culture. To inoculate the cultures, a certain concentration of spores in the inoculum is wanted (Spore count...
11 March 2021 5,111 8 View
I have prepared a liquid broth containing Yeast (S. Cerevisiae) that i need to add from it to fermentation media of lignocellulosic hydrolysate. I need to know based on what parameters do we add...
06 August 2024 662 1 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
Hello, So I have mouse brain tissue MS data from one of my predecessors and I have human serum data from the hospital that I collaborate with. We are trying to find markers for the diagnosis of...
11 July 2024 8,484 0 View
I want to make some yeast extractions with a protocol that uses Isopropanol and NaOH. So in the end i will have an azeotrope of Isopropanol and Water that comes from the cell and NaOH. Can you...
08 July 2024 9,201 2 View
The plasmid that was transformed originally is correct (sequenced). I am expressing different gene targets from the yeast genome to optimize my protein. When I sequence the incorrect size, it...
01 July 2024 2,356 6 View
Image attached is at 20X. Cultured media for checking presence of bacteria and yeast and both tests were negative.
27 June 2024 7,473 4 View
I need some articles on the analysis of the nutritional quality of barley (H.vulgare.L) and the analytical approach of metabolomics and proteomics.
26 June 2024 3,451 2 View
Hello, community, Could you please clarify whether current legislation permits the reuse of algae biomass after it has been used to treat non-hazardous decontaminated laboratory organic waste?...
05 June 2024 5,040 1 View
Hi everyone, I wanna do proteomics of monkeys serums in 2 states, healthy and patient. How many monkeys in each group I need to run proteomics and validate my data from it? Do I deplete albumin...
04 June 2024 4,091 3 View
I'm planning to perform BSA degradation using YCB and agar, but I need a protocol. Does anyone have one they'd be willing to share?
02 June 2024 6,885 8 View