A rapid quantitative measurement of accumulated polyhydroxyalkanoate (PHA) is essential for rapid monitoring of PHA production by microorganisms.For measurment of PHA  a 96-well microplate is used as a high throughput means to measure the fluorescence intensity of the Nile red stained cells containing PHA.  The linear correlation is obtained between intracellular PHA concentration and the fluorescence intensity represents the potential of the Nile red method employment to determine PHA concentration. All tested PHAs fluoresced maximally at excitation wavelength between 520 and 550 nm, and emission wavelength between 590 and 630nm. Polyhydroxyalkanoates (PHAs) are biopolymers. Waste frying oil is abundant and can be used in PHA production without filtration. Parameters Affecting Poly(3-Hydroxybutyrate) Quantification by Gas Chromatography. Biosynthesis and characterization of medium chain length poly-3-hydroxyalkanoates (mcl-PHA) produced by Pseudomonas putida Bet001 isolated from palm oil mill effluent.Depending on the type of fatty acid used, the 1H NMR and GCMSMS analyses of the chiral polymer showed a composition of even and odd carbon atom chain with monomer length of C4 to C14 with C8 and C10 as the principal monomers. No unsaturated monomer was detected. Thermo-chemical analyses showed the accumulated PHA to be semi-crystalline polymer with good thermal stability.

Hi you may used HPLC and GC analysis for quantification of PHA and its copolymers.

Nile red-based flow cytometry protocol is used to analyse the cell  Polyhydroxyalkanoates.

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Hello, we used a range of methods in this paper: Article Mass Spectrometry Reveals Molecular Structure of Polyhydroxy...