If we have an suspension culture of cells and If we have to check that that the cells in the culture are in which growth phase (lag,log, stationary or death) So,how can we check it?
After COVID-19 it has seen that EFL learners technological affiliation has raised. In addition, in the post-COVID period learners started to engage AI technologies like ChatGPT while learning...
08 August 2024 8,964 4 View
How we can cite the papers from ResearchGate. I am trying to create citations for this article, Quantum Machine Learning Algorithms for Optimization Problems: Theory, Implementation, and...
08 August 2024 6,688 3 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,621 1 View
I want to refine one XRD peak of my in-situ xrd but the background is never working good which ultimately fails the refinement. How to refine and adjust the background using GSAS-II
05 August 2024 5,290 2 View
Hi, i would like to simulate an absorption process in Aspen Plus. I want to use the NRTL model und would like to add some individual Henry coefficients. Is that possible and how?
05 August 2024 2,332 2 View
Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...
05 August 2024 3,782 2 View
AI tools like ChatGPT can enhance research work significantly when used responsibly and in conjunction with thorough human oversight.
05 August 2024 1,842 3 View
Have you ever seen a LC-MS/MS method uses both internal standards and external standards (in matrix matching purpose) but the concentrations of internal standards are outside the calibration curve...
05 August 2024 3,083 6 View
Hi everyone, I am working on brain slices for visualizing a protein in the soma and dendrites, using a fluorescence tag. However, I need a tool (not paid) for reconstruction of the whole neuron,...
04 August 2024 4,725 2 View
How to enhanced h-index in Research Gate?
04 August 2024 3,368 4 View
I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?
11 August 2024 3,115 1 View
I am trying to adapt adherent CHO-K1 cells in F12K+10%FBS to suspension culture in only F12K, but in vain. I have followed Mirus bio as well as Thermo protocols, please suggest any alternative....
31 July 2024 3,756 0 View
If the case of Irak is of December 2020 then i think I can suggest pl follow all the infection cause and death, already in our researchgate.
19 July 2024 3,356 2 View
I am currently culturing leukocytes from TLRE filters from our blood bank. I have been struggling to culture these cells, as well as improve their viability. I was wondering if anyone would be...
16 June 2024 3,752 0 View
Have you ever seen a small number of cells, 50-100 thousand, after centrifugation as a dry pellet in an Eppendorf tube without a microscope? Are there methods to potentially stain the cells in the...
09 June 2024 6,311 0 View
I am currently running Cell Death ELISAs, using the Roche Cell Death Detection ELISA kit. My samples are from the supernatant of bacterially infected primary canine cells. I am seeing no color...
02 June 2024 8,682 1 View
Impact on Iran’s Policies: Domestic: Raisi’s death may lead to shifts in Iran’s political landscape. Foreign Policy: Immediate changes are unlikely, as the supreme leader holds final...
20 May 2024 3,143 3 View
Hi all, I am trying to induce decidualization in primary human uterine fibroblasts. The problem is that when i use charcoal stripped FBS instead of normal FBS, it causes a massive cell death....
16 May 2024 6,990 3 View
I was wondering if you can move immunofluorescence ready sample that is in the form of suspension on 96-well plate to another well plate. Is that possible?
14 May 2024 596 1 View
Anyone tried adapting UMNSAH/DF-1 (Chicken Fibroblast cells) from adherent to suspension cells. Currently we are using Xerumfree TnC bio serum alternative with DMEM. The cells are not adapting...
05 May 2024 3,316 1 View