Hi, I am working on genotyping of human GCDH gene, would like to know all the possible ways i can predict a probable region where the promoter will be.
YOu can use this book as reference to do what you need to know also to do many other bioinfomatic analyses "bioinformatics for dummies" you can find your desired task directly in the table of component
If you know the sequence of your gene which will usually start with ATG at the 5` of your sequenced after the promoter region and followed by the ORF of your gene. There are many online tools to predict and determine the ORF (e.g. ORF finder) you can also predict your promoter using other software like PLACE . you can check the attached document which may help you for promoter isolation and cloning
Article The pJan25 vector series: An enhancement of the Gateway-comp...
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