I want to evaluate the toxicity of the compound according to OECD guideline in order to determine the safety dose level. However, the compound is not enough for acute and subacute toxicity evaluation. Is there any toxicity test that I can use to measure the safety level of compound? Can I just refer to in vitro cytotoxicity assay to determine the safety dose level?
Well in vitro toxicity can't substitute the in vivo experiments! If you dont have for both tests at least try performing one (acute), and try to give the high doses. If the toxicity is not observed and you dont have any more substance just say it like that in your work. Or try to find/isolate/buy more substance
I would do a ascending tolarability dose study in mice to get a PK profile and at least a hint for toxicity (1 male 1 female plus control)
In addition in vitro tests (cytotox, mutagenicity
It depends what you would like to investigate. Which is your target endpoint. Do you have an idea of the mode-of-action of your compound? Why are you interested in testing it? Because it is a contaminant or because you would like to use it eg as a drug. All of these questions are important to decide the best model for your case.
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