Recently it has been proven that GH61 group of enzymes belonging to CaZy group has shown to play crucial role in enzymatic saccharification. How can one detect LPMO activity in the crude cellulase extract?
Hi,
You need to identifiy oxidized oligosaccharides using ionic chromatography. Have a look at Bennati-Granier et al., Biotech biofuels 2015.
Jean-Guy
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