Articles describing the use of carbon dots in agriculture have been increasing recently. Again, studies have found that carbon dots size and shape and some other features are effective. Therefore, it is important to be able to produce carbon dots in the desired size range.

The carbon dots produced in laboratory scale are purified and classified using various methods (centrifugal, size exclusion chromatography, membrane filtration, dialysis bags, etc.). Since carbon dots need to be produced on an industrial scale for agricultural use, what should be the most appropriate and cost-effective method for large-volume production? (Carbon dots are produced from biomass and carbon dots are 3-6 nanometers in size. Only carbon dots with a size of 4 nanometers are required.)

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