Dear all,
I have flash-frozen mice brains for RNA sequencing and ATAC sequencing. However, in flash-frozen tissues isolating nuclei is problematic. And I can only use one side of the hippocampus, the other I will use for RNA seq.
Is there any optimized protocol that you use for nuclei isolation from flash-frozen tissue? Or shall I need to use at least 2 of the hippocampus to obtain enough nuclei? Or the worst case shall I use cortex instead which contains more cells.
But the most important part how can I isolate nuclei without disturbing nuclei from flash-frozen tissue?
I have ATAC-seq kit from cellbiologics cat: CB6936
Best Regards
Yağız
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