Nutrients, density of the inoculum, agitation, oxygenation, is it batch? feed-batch? continuous? density of the product, genetics of your inoculum

Look to the conditions of industrial penicillin production.

Dear Sambhaji,

feeding important precursors (especially amino acids with high anabolic costs) may help, like shown in the following publication:

https://www.ncbi.nlm.nih.gov/pubmed/22252649

Also protein folding, functioning and secretion might be improved by finding optimal process parameters (pH, T) and medium composition (e.g. addition of compatible solutes ("chaperones")).

Hope that helps

Michael

https://www.ncbi.nlm.nih.gov/pubmed/22252649

@ David E. Bautista-Erazo the process is feed batch fermentation, inoculam developement , optimum temperature & pH has been done for enzyme production.

thanks Michael Kohlstedt ...very much informative ....

I suggest you consider statistical design of the fermentation parameters using the response surface methodology. Good luck!

Thanks Robert.. We are using stat ease response surface methodology which are giving very promising results.. 

We are facing how to study the KLa ,oxygen uptake rate for fungus in production media containing high insoluble solids ...

Dear Sambhaji

I think if you can use the off-gas analyzer to monitor the exhaust CO2 and O2 gases during the fermentation, you can calculate the oxygen uptake rate and CO2 evolution rate easily. Please see the attachment.

Good luck