Which is best solvent and most suitable extraction method for plant phenolics?
Hi Dr. Reshama, solvents such as ethanol, methanol, acetone and ethyl acetate was reported to assist in the extraction of phenolics in plants. However, their efficiency might be different (due to many factors). One of the easiest extraction methods is the shaking water bath extraction by using 50% of ethanol as a solvent. We did this in our study for extraction of anthocyanins and determination of phenolics and other attributes in the mangosteen pericarp. I have attached the file here for your reference. Hope this will help.
This is one of the most difficult questions in the chemistry of secondary plant metabolites. As there are so many phenolic compounds of absolute variation in polarity, this clearly depends on the research question. Moreover, most of the methods described suggest to apply temperature in the extraction process. This might lead on the one hand to a certain degradation to smaller substances, but also on the other hand to a certain coloring, consisting of polymers such as melanins and melanin-like compounds.
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