DMSO concentration in cell culture? Precipitating while PBS is added?

I am testing a compound on RAW 264.7. the compound is soluble in DMSO. but to ensure that final concentration of dmso is not more than 0.1%, we are first dissolving it in dmso and then diluting it...

11 February 2021 1,136 3 View

ANSYS APDL RC Beam modelling?

Hello I have modelled RC beam in ANSYS apdl. first create volume, then create keypoints & create a line joining that keypoints to make reinforcement. I am working on thermal analysis, for...

20 January 2021 7,344 2 View

ANSYS APDL tabular entry related?

Hello I want to add thermal convection load on specific area of concrete volume. it asks for film coefficient which is a constant value but the bulk temperature has no. of values that i have as...

18 January 2021 6,627 1 View

What is the best way to maintain constant concentration of a volatile organic liquid in an open experimental set-up?

The purpose is to keep a NAPL (eg Toluene) concentration constant in an open tank set-up.

27 December 2020 7,325 2 View

How i can input temperature depedent stress strain curve for concrete & steel in ansys?

i am working on thermo-mechanical analysis of RC beam using ANSYS. i want to input temperature dependant stress strain curve in ansys. i tried using elastic > multilinear but a warning attached...

23 December 2020 9,928 2 View

What are some best features that can be added in IoT based pollution monitoring system ?

Actually I am developing IoT based pollution monitoring system and need some ideas which help in developing great product with great features

10 October 2020 5,657 7 View

Can LoRa Technology be used for managing underwater data center ?

As per the latest research, Microsoft finds underwater data centers are reliable, practical and use energy sustainably so how can we enable IoT with LoRa technology in the underwater data center...

05 October 2020 535 4 View

How can I calculate the concentration of nanoparticles being used in UV-visible absorbance scan?

I have taken 1000 PPM of nanoparticles in 1 ml of RO water and pursuing its UV visible spectro absorbance scan. They are showing respective peaks on the baseis of energy transition of electrons...

19 September 2020 6,276 3 View

How can Gold be extracted from mining concentrates?

I work at a precious metal refinery. We extract gold using aqua regia. However, sometimes Gold does not dissolve in AR. Sometimes it precipitates due to cold weather? Is there a way to make sure...

07 August 2020 7,059 3 View

Chemistry behind synthesis of boron nanoparticle?

Hello everyone, I want to know whole chemistry behind synthesis of boron nanoparticle via chemical and biogenic methods. Focussing on why naked boron is not used as I have seen in papers, boron...

05 August 2020 1,195 14 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

Uncertianiny in using dry or wet basis in mixing a sediment with fuel oil?

Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...

24 February 2021 9,839 3 View

Markers of mitochondrial activity in FFPE tissues?

Hello everybody, Does someone have any experience with assessing/extimating mitochondrial activity on FFPE tissues? Precisely, no TOM20, ATP5B, etc: we are trying to get an approximative idea of...

24 February 2021 4,618 4 View

Can a Golgi-stained dendritic spines picture processed by Image J be directly used for publication?

24 February 2021 3,348 2 View

Is there an appropriate CD8 antibody for IHC-P for staining human breast cancer tissues?

Hello, We are planning on staining Formalin-fixed paraffin breast cancer tissue sections (FFPE) with CD8 Ab via immunofluorescence (Tissue IF). Based on our existing protocol and imaging...

24 February 2021 9,578 4 View

Reagents for histology slides?

Dear all, I am planning to study histology and want to do that at home. I have only stained a blood smear with Giemsa but now want to try a wider range of animal tissues. What reagents will I...

24 February 2021 5,430 3 View