Hi everybody. Sorry, I forgot how is it possible to update my personal project, I want to add some new information to it. Thank you. Igor
Igor, Researchgate eliminated the Projects feature
https://www.researchgate.net/researchgate-updates/retiring-projects
Dear Colleagues, I have the following problem: I’m trying to amplify a cassette with a resistance gene (about 2 kb) from the yeast genome, so that I can then insert it into a plasmid. With Taq...
03 July 2024 105 11 View
Greetings, dear colleagues! Do you know any technique utilizing some ~ordinary molbiol lab reagents (not the commertial kits for isolated mitochondria staining) that will allow isolated...
07 June 2024 5,909 0 View
If Proven how Riemann hypothesis will help the development of AI and maybe help us create real advanced self-councious algorythms?
05 June 2024 9,787 3 View
We are working on gathering raw data and information about the scope/share of private donations and public funding to pilitical parties that sometimes in EU is a part of responsibilities of...
16 April 2024 5,178 0 View
I have a dataset of trace elements LA-ICP-MS analyses with raw data, which consists of a set of a single file for each spot analysis. Each of these files has some columns (masses) and plenty of...
24 March 2024 898 6 View
I am analyzing some time-series data. I wrote a script in R and used two methods from two different packages in R to calculate the DW statistics and respective p-values. Surprisingly, for the...
14 March 2024 6,127 1 View
Greetings. Probably the question is not complex at all, but can't find an answer. If I have RT-qPCR data of gene expression in a sample with multiple analitycal replicates - to compare it to data...
13 March 2024 7,640 1 View
In particular, I have few papers with spelling Agranovskii. Thank you
05 February 2024 3,764 1 View
I am using the grf R package (https://grf-labs.github.io/grf/reference/causal_forest.html) to obtain causal effect estimates for a continuous treatment variable. The package description says:...
15 January 2024 1,990 0 View
If we keep in mind that R^{4}=R^{+}xS^{3}=R^{3}*RP^{1} where * means a direct product with a singularity at the zero point of a 3-dimensional Euclidean space in which the projective line is...
30 December 2023 9,110 1 View
Hi, Could somebody please explain to me why lunar "soil" simulants JSC-1 and JSC-1A contain about 3% of ferric oxide while real lunar regolith do not have any ferric iron? Thank you, Igor Brown, PhD
26 May 2023 5,520 3 View
I am in the process to write a new research grant and I am thinking to replace my old EPC-10 (more than 15 years of intense use) with a new amplifier. I like the HEKA amplifiers, but dislike the...
10 March 2022 2,846 0 View
I have calculated the kd value for peptide (13 mer peptide) and LPS (Lipopolysaccharide) interaction through SPR and fluorescence displacement assay and got 1000 fold difference. the equilibrium...
20 October 2021 8,359 3 View
Dear colleagues - Could I be confident that adding 1% SDS (V/V) into TE buffer and boiling cultures in tubes for 10 minutes would kill any living cell? Thank you, Igor Brown
30 June 2021 3,984 6 View
Hello! Looking for informative maps of ice compression and vessel besetting in the Arctic area – to use in the presentation. Is anyone aware of such maps – for the whole Arctic Area or local...
20 May 2021 560 2 View
I'm recording local field potentials in fairly long (several minutes) continuous gap-free recordings. I've tried converting the data from .abf to .ibw with Stimfit, but the .ibw files are...
18 April 2021 9,905 3 View
Hello! This a special question to the naval architects / shipbuilders. Looking for some tentative estimations of the possible increase of the cost of the vessel built in accordance to be able to...
01 February 2021 8,905 2 View
Hello everybody! Looking for a good source of information on strong and extreme winds in the Arctic (monthly values, annual extremes etc.). The form of maps (with isolines) will be most...
25 January 2021 9,271 4 View
Hi folks, This is a software-related question. I've seen many researchers asking how to export data using Malvern Zetasizer software. Most people simply answer the most obvious suggestion: click...
27 August 2020 1,551 5 View
Dear colleagues, I am currently comparing some genomic features in different representatives of Fischerella genus isolated from different eco-types. Unfortunately, neither PCC nor UTEX collection...
11 July 2020 4,287 12 View