How can I understand how much of the carbohydrate, lipids and proteins of food waste (substrate) has been consumed in anaerobic digestion?

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Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

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How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

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Which database would give me this list of chemical components?

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Uncertianiny in using dry or wet basis in mixing a sediment with fuel oil?

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Anbarasan Tamilalagan

exactly.the microbial mass that goes in will consume the organics and will transform to more biomass.

total organics in = organics consumed for growth + unconsumed organics + organics consumed for maintenance

an estimation of the bio-molecules before and after certain intervals of time will give an idea for your query.

Marvin Gruhn

Hello Razieh, you may want to have a look at the methods of biochemical characterization presented in there publications:

Colorimetric method for determination of sugars and related substances. M Dubois, Ka Gilles, Jk Hamilton, Pa Rebers, F Smith . 1956. Anal Chem 28 350-356.

Protein Measurement with Folin-Phenol Reagent. Lowry et al. 1959. Biochemistry 193(1)

You can apply these to your substrate as well as your reactor effluent. It gives you an idea of how much carbohydrates and proteins have been consumed. But please keep in mind the biomass growth of the microbial consotria.

Razieh Rafieenia

Dear Marvin and Anbarasan

Thank you very much for your answers. You helped me alot.