How can I transport cell culture medium to another city?

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Does broad spectrum phage become narrow spectrum by time ?

In the host range analysis, I saw that a temperate phage that I isolated had an effect against a different strain, but later (2 years) it did not affect that strain. What could be the explanation...

25 June 2024 9,729 3 View

What is the less detrimental solution for pH Reduction for L-amino Acid Solution?

What is the most suitable solution for reducing pH of an solution which contains L- amino acid and peptide in a non-destructive way?

21 June 2023 8,833 1 View

The problem encountered in sequencing the lysogenic phage genome ?

Dear researchers, greetings to all of you There is a situation that is not in my field of study but that I need to understand for an experiment. I isolated lysogenic phage, performed many...

28 March 2023 2,081 5 View

How can I solve my SDS-PAGE problem after AS precipitation?

Hello everyone, After precipitating the proteins with ammonium sulfate, we performed SDS-PAGE. However, there was a problem with the gel running, we could not see any band. We did dialysis to...

13 February 2023 7,564 2 View

How can be dissolve unclog the metal capillary pipe?

The metal core capillary tubes of Electrospinning were clogged with PVA/chitosan polymer. We soaked it in hot water and acetic acid solution (%5), but there was no change. How can we dissolve this...

31 January 2023 7,371 4 View

The lysogenic phage genome I isolated was very small band value in gel electrophoresis ?

The lysogenic phage genome, which I isolated by the traditional phenol-chloroform method, appears small on 0.6% agarose gel electrophoresis. When I compare it with Marker, it is equivalent to 250...

16 January 2023 3,537 7 View

MCF-7 cells gives green fluorosence, contamination?

Hi all, I did DAPI staining for MCF-7 cells as a control (not treated with anything at all). They are fixed cells and stained with DAPI for 10 mins and washed with PBS three times. Please see the...

29 May 2022 7,607 3 View

How can i apply anode and cathode buffers at Tricine SDS Page ?

Hello, We will use Tricine SDS Page protocol to seperate our low kDa proteins. In this protocol, there are anode and cathode buffer but we can not understand how should we add these buffers ?...

29 March 2022 9,767 3 View

How to calculate dV/dD Pore Volume (cm2/g.A) in BET analysis?

In my results, there are values below-mentioned: - Pore Diameter Range (A), - Average Diameter (A), - Incremental Pore Volume (cm3/g), - Cumulative Pore Volume (cm3/g), - Incremental Pore Area...

25 March 2022 228 1 View

How can I remove sodium salt from drugs ?

Hello, I want to discard sodium salt from drug (Nafcillin) ? We want to Nafcillin without salt, insoluble form in water ? So, is there any technic that we can apply ?

25 October 2021 6,009 0 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is this a facetotecta nauplius?

This larva was captured using a plankton net in the Persian Gulf during the summer. I believe it may be a Facetotecta nauplius.

08 August 2024 3,746 4 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Good Day, can I ask for your help on the questionnaire that I want to adopt?

I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates

03 August 2024 3,505 6 View