How can I transfer a gene into mice skeletal muscle invivo?

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Simon N Waddington

Hi Ahmed,

Lentivirus, adenovirus or adeno-associated virus will do (as will electroporation if you have the equipment). You can buy vectors off-the-shelf from Vector biolabs, UPenn Vector Core, Virovek etc. Depends if you have a specific muscle in bind but you can inject any of these into TA (Tibialis Anterior) & get nice specific transduction.

BW, Simon

Sandip Chakraborty

You can please refer to the links below to obtain certain valuable informations:-

1. http://www.ncbi.nlm.nih.gov/pubmed/1690918

2. http://www.ncbi.nlm.nih.gov/pubmed/8494924

3. http://hmg.oxfordjournals.org/content/11/14/1615.full

4. http://www.nature.com/mt/journal/v4/n5/full/mt2001251a.html

Ahmed Kamaleldin Elfadl

Thank you so much