Ahmed Kamaleldin Elfadl

7 Questions 12 Answers 0 Followers

Questions related from Ahmed Kamaleldin Elfadl

What is localization of MyoD and Myogenin in an injured murine skeletal muscle using Immunofluoresence?

I m using immunoflourecense stain for detection of MyoD and Myogenin expression in murine skeletal muscle injury model, and I got cytoplasmic signal in most of regenerating myoblasts, should I...

24 January 2018 1,100 2 View

Can any one help me with explaining this gel electrophoresis result?

I had this gel electrophoresis result and I was expecting 480bp amplicon size in lane (2). However, I got a band size of 900bp in (2). Taking into consideration that I did not get a band for the...

27 June 2016 882 7 View

Primer design/ PCR/ Cloning (PGEM-T- easy vector) ?

How to design primers to amplify a particular sequence of a gene to be cloned into PGEM-T-esay vector?? How to include some nucleotides in the amplified gene to be complementary to the vector...

24 January 2016 3,693 5 View

How can I check for self complementary and hair pin formation in case of long RNA probes up to 1kb?

and what are the factors that I should take into consideration when designing long probes?

30 December 2015 4,799 3 View

How do I make probes highly specific to a targeted gene, if there are no 3'UTR or 5'UTR in the gene sequence as indicated by NCBI?

Note I am working with highly conserved gene among family.

29 December 2015 7,819 2 View

What is a good software to check primer dimer formation and hairpin loop formation?

online free software

20 November 2015 7,960 7 View

How can I transfer a gene into mice skeletal muscle invivo?

Should I use adenovirus or lentivirus for transfection? Providing me with a name of a kit will be highly appreciated.

08 October 2015 3,300 3 View