You can use normal PCR for standardization of your primer set and choose the best condition.  

Actually, I believe that correct primer design followed by validation is very important aspect of a qRT-PCR.

When you design the primers, take care in which region are you designing the primers (conserved or non conserved regions of the gene) depending on what do you want to test.

I would recommend you to first test the primers, for example using PraTo software (http://prato.daapv.unipd.it).

Then, when you are performing qRT you should use the melting curve analysis to confirmed specificity.

Hope it helps

You could also align the gene sequences of different fungal species then align your primer on your gene of interest and watch for specificity (or simply blast).

Hi,

Before your qPCR, you can test the best temperature for your primers, using a PCR and a temperature gradient, and then you choose the temperature that made the best amplification. And finally as Dubravka Cukrov said, you must use a melting curve and a dilution range for the primers efficiency

I hope I helped