I need to clone and purify 3 different BSH and I need to know if these are still active after the purification step. A would like to have a quick qualitative and quantitative colorimetric assay that can be performed with purified protein.
Not so much interest in substrate specificity at this stage.
Any suggestion?
A fluorogenic substrate for bile salt hydrolase is described in this paper:
https://www.nature.com/articles/s41598-018-37656-7
A substrate for a luminescence method was described here:
https://advances.sciencemag.org/content/7/6/eaaz9857
Here is a kit for measuring bile acids using a fluorometric assay:
https://www.bioassaysys.com/Bile-Acid-Assay-Kit.html?gclid=Cj0KCQjwpdqDBhCSARIsAEUJ0hNdW7bD1M0UbUm4JnElirAMscrLNo9RqdEdv0GN9a2FW64CLVmK1lAaAthbEALw_wcB
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