How can I store paraffin removed tissues?

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Why do I get low yield after rebinding of membrane protein?

Hi, I work with membrane protein and SDSL EPR. I use MTSL for labeling. My problem is I get very low yield after rebinding (from 5 mg/ml to 0.8 mg/ml) which is not enough to downstream work. I...

31 December 2018 8,888 0 View

What is the different between Column volume and bed volume in Ni-NTA gravity column?

Hi, I am working on Ni-NTA gravity column chromatography to purify my protein, I am confused with the different between column volume and bed volume. What is the different between those two?...

07 August 2018 7,546 3 View

How to remove unwanted protein in expression?

Hi, I am working on membrane protein which have his tag (recombinant protein) I express my protein in M9 media using BL21 (DE3) codon plus RP cells. I always get some extra band around 23KD...

04 May 2018 2,049 11 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Image Processing and classification using keras/Tensorflow?

Do you have some recommendations approaches on Image processing and classification of plant diseases using TensorFlow/Keras?

02 March 2021 1,493 4 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Which SAR dataset to use for generating coherency matrix and performing target decomposition (Description) ?

Working on chandrayaan-2 DFSAR data, there are three datasets available: 1) Slant range image data product: The slant range complex image file. Each pixel is represented by two 4-byte floating...

02 March 2021 8,481 3 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View

Duaa Dakhlallah

I snapped freeze them in liquid nitrogen after the use immediately and stored in -80 freezer .

Mark J Eagle

Snap freezing and then storing at -80 or liquid nitrogen temperatures are your best bet. However fixed and embedded tissues have poor yield and fragmented RNA.

Joao Paulo Rodrigues Marques

The tips of the researchers above are right. You can also make some thicker sections. To try to get a large amount of tissue and consequently RNA.

Eunice Mejia

I suggest you get new tissue and fresh freeze it. Can you really trust your RNA results after you have treated with any kind of fixative?

Fathima Dhilhani

Thank you for all of your suggestions