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Hi, I work with membrane protein and SDSL EPR. I use MTSL for labeling. My problem is I get very low yield after rebinding (from 5 mg/ml to 0.8 mg/ml) which is not enough to downstream work. I...
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Hi, I am working on Ni-NTA gravity column chromatography to purify my protein, I am confused with the different between column volume and bed volume. What is the different between those two?...
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Hi, I am working on membrane protein which have his tag (recombinant protein) I express my protein in M9 media using BL21 (DE3) codon plus RP cells. I always get some extra band around 23KD...
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