Does anyone know how to store MPTP? I just buy 10mg powder MPTP from SIGMA. And the product information says that the aqueous solutions may be stored for 24 hours at 4 ° C.
However, I need 0.1mg of it dissolved in 1 μl saline in each experiment. If I dissolve all the MPTP in saline at once, I am afraid after 24 hours the left MPTP can not be used any more.
So I wonder very much how to store MPTP, should I have to divide the powder MPTP into 100 pieces and dissolve it in 1 μl saline each time before each experiment?
Does anyone can help me, thank you very much!
I agree with Kate, powder should be stored at room temperature. However, I would never try to open the vial to take a little quantity of powder since it is highly toxic, under the hood you could spread it all over. The manufacturer sell the product in a closed vial just to add saline and dilute the product. I propose you to dissolve the whole vial and make aliquots and store at -20ºC. We used for monkeys and storing at -20ºC worked (just thaw once).
Thank you very much for all of your kindly help! Kate is right, the product number of MPTP I mean is exactly M0896. Since the whole stuff is only 10mg and its highly toxic, it is relatively hard to make a little solution at a time.
Further more, thank you for Salvador Sierra's great help, I will follow your advice, thank you very much!
A structural correction to Dr. Sharma's: MPTP is not a tetrahydroisoquinoline, it is what its name says--a pyridine. There are tetrahydroisoquinoline alkaloids that might resemble MPTP's structure a little, but the closer resemblance are the tetrahydro-beta-carbolines, which are more or less superimposable on MPTP (e.g., see Brain Research, Vol 675, p. 275-288 (1995)), because of an indole nitrogen bridge.
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