How can I stimulate endothelial and smooth muscle cells to release exosomes in vitro?

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Refik Kanjhan

Hi Mario,

Are those cultured cells? I would think electrical stimulation at single cell level or field stimulation should facilitate release of exosomes. Another possibility is raising extracellular K concentrations.

best wishes, Refik

Muttuswamy Sivakumaran

Yes, potassium chloride accelerates exosome release. Some people have tried chloroquine as well

Mario Grossi

Thanks a lot ;-)

Do you mean 60mM KCl for how long time? And it should be in presence of 10%FBS?

Thanks in advance

Published reports suggest 30mmol/l for 30 mins.