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Questions related from Mario Grossi
I have 2 rat experimental groups. Their weights were taken before and after 17-18h starvation. Group1 showed less body weight loss and a tendency of increasing of blood glucose level compared to...
15 September 2017 4,653 3 View
The skeletal muscle cells accumulate glycogen. Would the increase in glucose uptake trigger an over production of glycogen granules and this, in turn, push the mitochondria close to the nuclei?
09 September 2017 9,352 1 View
Dear RG-friends, I would like to check if there are any DNA fragmentations following treatment in primary cells. I know that the comet assay would be best, and since we do not have it at moment, I...
29 July 2017 4,571 2 View
Dear RGfriends, I would like to stain primary cells against Ki67-antibody (and more antibodies) at different cell passage (from P=1 to P=6). In order to run the ICC for all samples at the same...
12 May 2017 6,271 10 View
It was not possible to see any expected band for the target, with a very low molecular weight (8-12 kD), by WB. The membrane was very clean after incubation with Ab-I-AbII. Probably we lost...
03 March 2016 6,294 3 View
Please, Could you tell me what is the best condition (cell density/media..)to freeze and to culture human T-cells? How long can T-cells be in culture? Thank you in advance Mario
05 February 2016 1,306 3 View
There are paper where is written that "a protein is less express in vitro compare to in intact tissue", such as caveolins. The epitope is recognized by antibodies by its linear sequence of amino...
24 January 2016 5,133 3 View
