Mario Grossi

4 Questions 16 Answers 0 Followers

Questions related from Mario Grossi

Would it be possible to directely load cell pellets in agarose gel electrophoresis

Dear RG-friends, I would like to check if there are any DNA fragmentations following treatment in primary cells. I know that the comet assay would be best, and since we do not have it at moment, I...

29 July 2017 4,562 2 View

Would it be possible to store 4%PFA fixed cells in PBS at 4degrees for many days?

Dear RGfriends, I would like to stain primary cells against Ki67-antibody (and more antibodies) at different cell passage (from P=1 to P=6). In order to run the ICC for all samples at the same...

12 May 2017 6,262 10 View

Can Dot Blot be of help when Western Blot does not work for certain Abs?

It was not possible to see any expected band for the target, with a very low molecular weight (8-12 kD), by WB. The membrane was very clean after incubation with Ab-I-AbII. Probably we lost...

03 March 2016 6,282 3 View

Freezing and culturing Human T-cells?

Please, Could you tell me what is the best condition (cell density/media..)to freeze and to culture human T-cells? How long can T-cells be in culture? Thank you in advance Mario

05 February 2016 1,293 3 View