Mario Grossi

10 Questions 27 Answers 0 Followers

Questions related from Mario Grossi

Body weight and blood glucose level?

I have 2 rat experimental groups. Their weights were taken before and after 17-18h starvation. Group1 showed less body weight loss and a tendency of increasing of blood glucose level compared to...

15 September 2017 4,715 3 View

Any one has experience with skeletal and cardiac muscle cell isolation from mouse E18.5 embryos ?

Dear RG-friends, I would like to ask tips how to take cells from heart and muscle of mouse E18.5 embryos. I hope to get an answer as soon as possible. Thank you so much in advance. Best Regards, Mario

11 September 2017 1,779 0 View

Do the glycogen granules push mitochondria close to the nucleus?

The skeletal muscle cells accumulate glycogen. Would the increase in glucose uptake trigger an over production of glycogen granules and this, in turn, push the mitochondria close to the nuclei?

09 September 2017 9,412 1 View

Would it be possible to directely load cell pellets in agarose gel electrophoresis

Dear RG-friends, I would like to check if there are any DNA fragmentations following treatment in primary cells. I know that the comet assay would be best, and since we do not have it at moment, I...

29 July 2017 4,633 2 View

Would it be possible to store 4%PFA fixed cells in PBS at 4degrees for many days?

Dear RGfriends, I would like to stain primary cells against Ki67-antibody (and more antibodies) at different cell passage (from P=1 to P=6). In order to run the ICC for all samples at the same...

12 May 2017 6,338 10 View

Dimers of Pyk2?

Hej, I am looking for papers/gels (bands 220-250 kD) where I can see the dimers of Pyk2. Anyone can tell me more about the dimerization of Pyk2 and what could drive a dimerization of this...

09 April 2016 7,589 2 View

Can Dot Blot be of help when Western Blot does not work for certain Abs?

It was not possible to see any expected band for the target, with a very low molecular weight (8-12 kD), by WB. The membrane was very clean after incubation with Ab-I-AbII. Probably we lost...

03 March 2016 6,356 3 View

How can I stimulate endothelial and smooth muscle cells to release exosomes in vitro?

Hi. I would like to stimulate ECs/SMCs to release exosomes in vitro. Do you a good protocol or tip to getting started? Thank you in advance.

16 February 2016 8,328 5 View

Freezing and culturing Human T-cells?

Please, Could you tell me what is the best condition (cell density/media..)to freeze and to culture human T-cells? How long can T-cells be in culture? Thank you in advance Mario

05 February 2016 1,364 3 View

Does the epitope lose its conformational structure in vitro sistem which leads to a less affinity against the antibody?

There are paper where is written that "a protein is less express in vitro compare to in intact tissue", such as caveolins. The epitope is recognized by antibodies by its linear sequence of amino...

24 January 2016 5,184 3 View