I have three dose-response curves (concentration vs TEER (% on blank)), coming from three different passage ranges of the same cell line. I have fitted the three curves using OriginPro and each curve has its own fit. I want to see if there are statistical differences between the dose-dependent response in the three different passages of the cell line. I could perform a t-test between the points of the curve, but I would lose the informations coming from the fit (apart from the EC50). Should I use a two-way ANOVA test?
Thank you in advance
OK. I was glad to see this question in the ResearchGate. Dose Response. my favorite subject. The fact is that comparative analyzes such as T and ANOVA are not suitable for this task. Rather, the correct way is to compare curves and parameters such as EC50 and IC50. The best software to do this is the GraphPad Prism, version 8 of which has just been released. If you would like to send me your data.
I send a short video on how to work with the GraphPad.
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