How long can we consider that the data obtained with qualitative social research methods are "current"?

I cannot find any reference that deals with the temporal validity of qualitative data. I think there is a gap in the methodological literature on the temporal validity of the research results and,...

05 February 2021 9,788 20 View

Can I use the images of Collection 1 Level 2 Landsat 5 TM / Landsat 8 OLI to perform vegetation indices right away?

I'm aware that in order to perform any vegetation index like NDVI, SAVI or OSAVI you need to do some atmospheric, radiometric and terrain corrections. I'm pretty sure that Level 2 images from...

21 December 2020 4,088 3 View

BSEM/SEM approach?

Hi all! I'm getting into BSEM approach and I am specially concerned about when it is recommended or not to use this model. I have tested a BSEM with a small sample obtaining excellent results,...

07 September 2020 3,919 3 View

Unable to perfiorm raster to-table join in ArcGIS?

Dear all, I am trying to replicate some results from a publication. There, one of the datasets they use is WISE30sec map (available for download here:...

18 February 2020 6,906 3 View

Low 260/230 ratio using Qiagen DNeasy kit?

Recently I performed a DNA extraction using Qiagen DNeasy kit and the results show a 260/230 ratio of 1.05 likely due the presence of phenol or other organic contaminant... I have read that you...

16 February 2020 787 3 View

Why is the TMB substrate turning blue without being in contact with the sample and in the dark?

Hi everyone, last time I was doing an ELISA without exposing it to the light and before I added the substrate it was turning blue. Do you have any idea or suggestion of a better substrate?, the...

03 January 2020 1,294 7 View

How data is normalized in Transcriptome Analysis Console (TAC) Software?

I'm using TAC software 4.0.2 (Affimetrix) for the first time to analize differential gene expression in two groups of samples (control and treatment). I have problems with the normalization step...

18 November 2019 7,146 3 View

Does anyone know of a practical energy efficiency model for wireless sensor networks (WSN) or software that uses attributes of that model?

I have already found some reference authors who model some parts of the system, for example, sensor, transceivers, link, propagation space, the network itself and, sometimes, putting it all...

11 November 2019 2,111 3 View

What is the minimum level of energy that allows a near body device to be long term self-sustainable?

My concern is about Energy Efficiency Model for WBANs. In a wide sense, I'm looking for ways to model the whole network in terms of power and energy. How much average power is required to keep...

11 November 2019 5,053 4 View

Why Bluetooth, or more recently BLE, is today's dominant technology for communications on sensors and IoT devices?

Is that correct? Could you give a list of pros and cons when compared to a plethora of options? For instance, IR-UWB is simpler (hardware mainly) than narrowband options, although it works with...

11 November 2019 4,370 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

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02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View