Calcofluor-white is known to stain beta 1-4 glycosidic bonds of cellulose and chitin. I want to visualize the chitin rich organs in C. elegans.
I have a bacterial protein 'X' and I want to check if it interacts with any host protein (Human/Mouse). Is there any tool available that can predict interspecies interactome, like how STRING...
08 September 2019 2,745 4 View
Hi, I need to check unknown proten-protein interaction by Gel shift assay, but i dont know the interacting partners. So, my questions are as follows: 1. What kind of transfer buffer and membrane...
03 April 2019 638 4 View
As far as I know, brilliant green is a basic dye belonging to aniline group containing dyes. Malachite green is also a basic aniline dye, which is used in endospore staining. In endospore...
10 November 2016 6,237 4 View
I was thinking of removing the excess bacteria by adding gentamycin to the harvested suspension. but again, the worms might take up the antibiotic and that can kill the bacteria present in the gut...
04 May 2016 8,110 4 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View