How to predict if there are any interactions between eukaryotic proteome and a particular prokaryotic protein?

I have a bacterial protein 'X' and I want to check if it interacts with any host protein (Human/Mouse). Is there any tool available that can predict interspecies interactome, like how STRING...

08 September 2019 2,593 4 View

What kind of transfer buffer, membrane, transfer condition and antibody staining is appropriate for Native PAGE WB?

Hi, I need to check unknown proten-protein interaction by Gel shift assay, but i dont know the interacting partners. So, my questions are as follows: 1. What kind of transfer buffer and membrane...

03 April 2019 510 4 View

Can anyone tell me how does brilliant green inhibit Gram positive bacteria selectively?

As far as I know, brilliant green is a basic dye belonging to aniline group containing dyes. Malachite green is also a basic aniline dye, which is used in endospore staining. In endospore...

10 November 2016 6,025 4 View

How can I check the bacterial load in C. elegans?

I was thinking of removing the excess bacteria by adding gentamycin to the harvested suspension. but again, the worms might take up the antibiotic and that can kill the bacteria present in the gut...

04 May 2016 7,992 4 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View