Kasturi Chandra

5 Questions 7 Answers 0 Followers

Questions related from Kasturi Chandra

How to predict if there are any interactions between eukaryotic proteome and a particular prokaryotic protein?

I have a bacterial protein 'X' and I want to check if it interacts with any host protein (Human/Mouse). Is there any tool available that can predict interspecies interactome, like how STRING...

09 September 2019 2,682 4 View

What kind of transfer buffer, membrane, transfer condition and antibody staining is appropriate for Native PAGE WB?

Hi, I need to check unknown proten-protein interaction by Gel shift assay, but i dont know the interacting partners. So, my questions are as follows: 1. What kind of transfer buffer and membrane...

04 April 2019 592 4 View

Can anyone tell me how does brilliant green inhibit Gram positive bacteria selectively?

As far as I know, brilliant green is a basic dye belonging to aniline group containing dyes. Malachite green is also a basic aniline dye, which is used in endospore staining. In endospore...

11 November 2016 6,167 4 View

How can I check the bacterial load in C. elegans?

I was thinking of removing the excess bacteria by adding gentamycin to the harvested suspension. but again, the worms might take up the antibiotic and that can kill the bacteria present in the gut...

05 May 2016 8,073 4 View

How can I specifically stain only the chitin present in the phrarynx and eggshells of C. elegans? Is it possible with congo red or calcofluor-white?

Calcofluor-white is known to stain beta 1-4 glycosidic bonds of cellulose and chitin. I want to visualize the chitin rich organs in C. elegans.

05 May 2016 1,244 0 View