We started experiencing peak Tailing after over 1000 successful injections with the column, We were in the middle of analysis of caffeine in Turkish coffee when the problem started at some point during optimization of extraction conditions. We have used the standard protocol of regeneration by reversing the column and washing with solvents of different polarity (Methanol, ACN, Isopropanol). Also with Pure ACN at 80 0C. We have also tried adding up to 2% TFA (Trifluoroacetic acid) and up to 2% TEA (Triethylamine) in the Mobile Phase but there was no improvement in peak symmetry. Is there any possible solution to this problem please? Thank you.