I would like to seperate oil from baked product with cloroform and petroleum ether (1:1, v/v ) fractions. I am not sure how can i apply it. Could you explain? How many volume i should add this solution on my sample. For example i weighted 2 g sample in a falcon tube and then add to cloroform-petroleum eter (30ml solution) and shake it then seperate it from falcon tube. Is ıt right ?
And after i obtain defatted extract, doing antioxidant activity analyse is giving true score?
What you are doing is right, the right comparison is no specific benchmark, which is clearly based on the condition of the samples, where samples must be dissolved by using a suitable solvent, however, need to be repeated to ensure that all the fat that is present in the sample extracted entirely
Chloroform should not be used to produce a product intended to be used as food or feed.
You can use ethanol or methanol by mixing with Ultraturrax appoximately 5 minutes and then centrifuge.I performed that procedure for phenolic content determination in extruded products and results correlated with literature.
To make sure that all the lipid is extracted from the sample matrix, soxhlet extraction using petroleum ether or diethyl ether for about 16 hours is a better option than shaking in a falcon tube.
Well, I don't know exactly hows the matrix but, if you'd like to obtainthe oil fraction from a powder-like product as a GRAS extract, you might try SFE (Supercritical Fluid Extraction), using supercritical CO2 as extracting solvent. You'll need to eliminate most water prior to extaction.
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