Fabio Pamieri

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Questions related from Fabio Pamieri

How can I resuspend homogeneously a bacterial pellet for CFU counting?

We are currently culturing several bacterial strains which grow as clumps in liquid medium (without agitation because they are micro-aerophillic) for CFU counting at a given OD (0.8 for instance),...

21 September 2023 8,804 1 View

Can I use a CellBIND flask to enrich for monocyte by adherence?

Hi, I want to enrich for monocytes by adherence from PBMC. I have CellBIND flasks or TC treated. What is the best? Then to detach them, I saw a lot of protocol. What is the best? Thanks a lot for...

02 July 2019 8,556 3 View

Imaging monocytes, macrophages & dendritic cells with antibodies

Hi all, I'm working with immune cells and lung epithelial cells and I will differentiate the monocytes (HPBMC) into monocyte-derived macrophages (mo-Mac) and dendritic cells (mo-DC). I would like...

01 January 1970 4,974 8 View

Quantification of monocytes, and derived cells, after transepithelial migration

Hi everyone! I am planning to do an experiment of transepithelial migration of monocytes (THP-1), and derived cells (monocytes-derived macrophages and dendritic cells), through an air-blood...

01 January 1970 6,410 0 View