How can I resolve this phage genome assembly into two separate genomes?

More Emma G. Piligrimova's questions See All

Looking for articles that provide guidance on doing Thematic Analysis (Braun & Clarke) as a team?

Does anyone know of any published articles that provide guidance on using TA with multiple team members? I am aware of one (see below), however, there are some disagreements in terms of some of...

23 July 2024 7,819 5 View

Is it possible to form a cell monolayer on top of Matrigel within an invasion assay?

I am currently using transwell inserts with the addition of 50 uL of matrigel for an invasion assay with various osteosarcoma cell lines. I have tried various dilutions of matrigel and all result...

17 June 2024 8,695 0 View

Interpretation of simple mediation analysis - individual paths or overall indirect effect that matters?

Hello, I would really appreciate some help interpreting the output from my simple mediation analysis using PROCESS macro in SPSS please. For context, the X predictor is severity of nausea and...

13 June 2024 9,597 3 View

How to know if RNA extracted from the desired brain area?

Hi! I am trying to do RNA extraction from p7 mice's somatosensory cortex and hippocampus. But I am not sure if the RNA was extracted from the correct brain region. I was wondering if there are any...

25 February 2024 5,484 1 View

Is it ok to use IP lysis buffer instead of RIPA for Western Blot?

Hi everyone, I transfected HEK cells with a protein that is intracellularly expressed through flow. Then I isolated the protein using RIPA buffer protocol, however, I accident added IP buffer...

15 February 2024 8,609 3 View

How long do BMDMs take to migrate through the endothelial monolayer in a transwell transmigration assay?

I need to perform a transwell assay on murine BMDMs (bone marrow derived macrophages). I am not sure how long I need to leave the macrophages in incubator to allow the migration. I am finding in...

30 January 2024 2,959 0 View

Is it possible to republish an already published article by chancing the used appellations into currently new defined scientific terminologies?

I already published an experimental research paper entitled "Neuroanthropology: Olfactory Recognition of the Self / Non-self by the Ancestral MHC: An EEG Study" in 2012 (doi:10.5539/ijb.v4n4p1)....

07 January 2024 2,508 4 View

BPA dissolution in water or solvent?

I came across a paper on the exposure of zebrafish to BPA at realistic environmental concentrations and I found no mention of any solvent used to dissolve the BPA. Instead, the exposure was...

09 November 2023 732 1 View

Are these bands too smeared for good qPCR results?

Below is a picture of a 1% gel of my qPCR products from salt marsh sediments (I know, its not the best picture). Standard and NTC are also labeled. I am wondering if my bands are too blurry to be...

12 October 2023 2,739 0 View

Weird shape of IPSCs, seeking suggestions for troubleshooting?

I'm doing patch clamp recording in Substantia Nigra pars reticulata(SNr), while I put ChR2 in D1-MSN neurons. During light stimulation, my elicited IPSCs has weird shape of IPSCs. How should I...

29 August 2023 3,107 10 View

How to improve plaques in Double Layer Agar Assay?

Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...

06 August 2024 5,801 4 View

Who of all the Global Scientific community will help me Prof. Dr. Yoshida make way for TPEOM, MEC ~EMC to return the atmospheric gases to the norma ?

TEP presentation caption (The Environmental Project) Re: Why should Washington’s DC, or any country government point of location think of as nowadays of as to being 'tomorrow as to come! if it...

03 August 2024 2,484 1 View

How is the bacterial genome's high protein count verified as genuine despite 800+ contigs and good metrics (98.55%completeness, 0.68% contamination)?

Given that the bacterial genome has over 800 contigs, but its quality metrics are good, with a completeness of 98.55% and a contamination of 0.68% as assessed by CheckM, what specific validation...

01 August 2024 1,514 1 View

Recovery Viurses from bacteria genome?

Hello everyone, I am currently looking for tools to recovery viral genomes from bacterial genomes, not metagenomes. However, I have only found tools that are designed for retrieving and studying...

28 July 2024 8,953 1 View

Why my negative control siRNA is decreasing the target gene's expression?

Hi Everyone, I'm using an siRNA kit to knock down a target gene. The kit guarantees that the negative control doesn't target any sequence in mouse genome, and when I use BLAST I don't find any...

23 July 2024 2,673 6 View

What are the strategies to Enhance IgG-Producing Plasma Cells in mice for Monoclonal Antibody Development?

I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...

22 July 2024 9,160 2 View

How to use NCBI datasets ?

I have been trying to extract genome from NCBI using their dataset tool, however some examples seem not to work : ./datasets download genome taxon "Homo Sapiens" --annotated --assembly-level...

20 July 2024 1,339 2 View

Enquire about the calculation of percentage of reads mapped to the reference sequence?

Hello researchers, I have sequenced the A549 cell line using RNA sequencing approach and then I have it against the human genome reference sequence. Would I expect a 100% mapping percent? I got...

20 July 2024 9,705 0 View

Promotor observation in region annotation of RNA RIP-seq?

Hello all, I extracted RNA from my samples and performed RIP-seq. After annotating the genomic regions using R, I obtained promoters, exons, introns, and UTRs. Given that my samples consist of...

18 July 2024 1,579 2 View

Where can I get PCR Primers for COI barcoding of birds?

We are in need of primers for a PCR protocol for chicken genome. We are looking at the COI barcode, 648-bp fragment of the 5' end of COI. Specifically Primers FalcFA, BirdR1, CO1_ExtF,...

16 July 2024 118 2 View