I have two carbon peaks of my sample that are superimposed with DMSO peak in carbon NMR. I got to know of this from HSQC and HMBC. Is there any way that I can bring them out ie change their shift so that I can see them. Compound dissolves in DMSO only.
Dear Clemens Anklin, But the DMSO peak has a quaternary carbon as well, seen in HMBC only. I am interested in this particular carbon. Will a DEPT help me with this cause I will not see a quat in DEPT it right? What if I run a APT experiment with a relaxation delay of 1/2J. Will this remove all my other carbons along with the methyl group of DMSO and just the quats remain?
If they have proton attached, you should try try with 2D-HSQC, otherwise with HMBC.
Good luck
A simple way it to run a spectre of dmso alone, scale it so it fits the dmso signal in your sample and subtract them.
Assuming that the 13C peaks that you are looking for do not have the same relaxation rate as the DMSO-d6 signal, you can try a 180°-delay-90° inversion-recovery experiment and choose the recovery delay such that the solvent signal is nulled. You will then see your signals, albeit not with their original intensities. (Admittedly, this is an experiment of the old school.)
- Badges
- Science method
- Similar topics
- Chemistry
- General Biochemistry
- Biochemical Techniques
- Analytical Chemistry