How can I reduce the incoming flow in slice chamber?

05 October 2017 1 7K Report

I am using a 4 channel slice chamber. The flow of aCSF to one of the channels is more than the outflow. As a result, the channel overflows and I lose my slice. Does anyone have any suggestion on how I can reduce the incoming flow?

Thank you,

Albertino Bigiani

I don't know the design of the chamber, but if you use a gravity-driven perfusion system, perhaps you can lower down a bit the solution reservoir until you get a perfect match with the outflow rate.

Albertino

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