Can anyone can provide a simple and straight method for measuring the plasma insulin concentrations in mice?
Recommend either the Alpco or Millipore mouse insulin ELISA Kits.
You can also use the Millipore RIA kit if you are equipped. Results from the ELISA and RIA correlate well.
The simplest way is to use ELISA. As for mice you need measurements in a small volume I would recommend Mercodia Ultrasensitive Mouse Insulin ELISA (Cat Nr 10-1249-01) which can deal with 10 microliters.
Hi All,
I have done a manual in-house competitive binding insulin I-125 assay (the best Gold standard assay I have ever used for Ins analysis) for quick results - takes about 20 minutes. We used this for patients undergoing surgery for insulinoma.
I have also used the LINCO elisa assay. You can get a result reasonable quickly esp if you are only after a low or high number and don't want to use the whole std curve.
I am about to test out a new kit from Alpco - the Stellux platform - a chemiluminescent EIA - let you know how it comes out. It has a 2 hour primary incubation and then you wash, add the chemiluminescent reagent and read.
I have used Millipore EIA and RIA, Crystal Chemical EIA and Mercodia all with good results.
If you are doing your research in China, you might have difficulty. We had a colleague who returned to China and found that she was unable to measure insulin perhaps due to poor handling of kits in transit and her workplace did nt have a way for her to use RIA. She ended up sending me the samples.
I agree the Mercodia Ultrasensitive Mouse Insulin ELISA there are de Bether, but you need to be very careful whit the standart curve.
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