Hello

I would like to quantify the protein content in Extracellular Vesicles (EVs) using ELISA. However, my attempts have yielded negative results. I am unsure about the next steps to take, particularly considering that my EVs are diluted. I believe this dilution may be causing an issue, especially since they were further diluted when the diluted/blocking reagent was added. What course of action would you recommend in this situation?

Should I lyse the EVs?