Inducing sporulation on rice tissue

Samples showing typical symptoms usually need to be placed into a humid chamber to induce sporulation. Greer and Webster (2001) successfully induced sporulation on both panicle neck nodes and leaves by placing the samples on "three pieces of moist filter paper in glass petri dish and incubated for 48 hours under continuous fluorescent lighting at room temperature".

Thanks, but I need to make a pure cuture of

Magnaporthe oryzae pathotype triticum from wheat samples.

Follow protocol described in:Article Cultural and morphological studies on Ponnampet leaf and nec...