How can I preserve the non myelinated axon in corpus callosum of brain, as i did the perfusion procedure but I lose the unmyelinated axon?

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How can I measure Audit risk assessment?

I am doing research, and one of my research variables is audit risk assessment. I want to do applied studies instead of questionnaires.

18 March 2024 9,997 3 View

Image j for western blot ?

Hi 👋 i use image j to analyze my band of western blot previously i select band then analyze it by plotting curve and calculating area under curve .. recently when i analyze band this appear with...

27 May 2023 5,190 8 View

Image j for western blot ?

25 May 2023 2,923 2 View

Colocalization analysis used fluorogram BIOP JACoP plugin?

Hi All, Recently, I've used BIOP JACoP plugin to analyse the protein protein colocalization during mitosis (mitotic cells, treated with Eg5 inhibitor). I struggle to understand the output of the...

21 May 2023 3,289 0 View

Western blot sample storage ??

I should do western blot experiment for detection of protein in liver sample .. i wash tissue with pbs then homogenize it with ripa lysis buffer and PMSF and Na3VO4 and then centrifuge it and...

27 February 2023 6,967 2 View

KI immunohistochemistry for mice brain?

I need to stain the hippocampus of mice brain with KI antibody. I tried different dilution of AB and retrieval with EDTA and citrate. But i still get week positive result. What can i change to...

01 February 2023 4,910 1 View

Melting temp of rtpcr ??

I do run for 2 sample real time pcr .. their melting curve for one sample show not available tm what this mean ??? Its ct value is 31 thanks alot

27 December 2022 4,640 1 View

How to make rdkit drawing visible in pycharm?

Alsallam alaykom. I am using rdkit and py3dmol in pycharm, where I faced problem in making drawings visible. it gives me for rdkit drawings: and for py3dmol drawings gives nothing. In jupyter...

13 December 2022 9,642 1 View

Do anyone have Morgagni FEI 268 transmission electron microscope. Grid holder??

Dear researchers.i really need help Do anyone have Morgagni FEI 268 transmission electron microscope. Grid holder?? Our grid holder has broken and alot of patients are waiting for their results....

02 December 2022 5,546 0 View

Question about purification of RNA ?

I do isolation of RNA from liver rat using genezol triRNA purekit geneaid company when i measure conc of RNA by nanodrop this ratio appear what does it mean ?

08 October 2022 4,356 3 View

How can I prepare virus for a TEM or SEM imaging?

I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?

11 August 2024 3,115 1 View

1. If I can quantize the atom using this hyperbolic spiral and classical physics, could nature do the same?

If we map as a continuous motion an ionising electron (beginning its journey at n=1) in an H atom, a specific hyperbolic spiral appears (see animation). When we solve this spiral formula, we find...

07 August 2024 5,343 2 View

Is there anyone with experience in TEM analysis who can assist with a manuscript for an upcoming journal?

Hello dear colleagues, We have prepared a manuscript on NiTi-based alloys and are seeking a second opinion on our current TEM results. If you are a Ph.D. holder with experience in TEM and have...

07 August 2024 9,563 0 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

Which distribution type should I use when calculating the average particle size from TEM image? and how to calculate the error ?

average particle size calculation from TEM

04 August 2024 2,921 1 View

Why reactivity isn't increased with more empty spots in valence shell?

If from a geometric perspective the non-halogens, non-noble gases have more empty spots in their valence shell, and the filling/exiting of any of the empty spots in the shell constitutes a...

03 August 2024 4,787 2 View

Why is the molecule's orientation with an electric field affect polarizability?

Why is the molecule's orientation with an electric field affect polarizability? Electrons are diffuse enough to be independent with respect to orientation and effect of electric field on...

03 August 2024 7,843 1 View

Why doesn't chromium 2+ ion use all its d-orbitals to receive lone pairs from six waters in [Cr(H20)6)]3+?

I'm guessing it's because the ligand experiences too much electron repulsion or proton repulsion from the chromium to insert them close to the 3d-orbitals which are close to the metal nucleus. Is...

03 August 2024 1,370 1 View

Is it necessary to covary exogenous constructs in a structural model?

I am working on a SEM model where i have 7 latent variables (6 exogenous and 1 endogenous). In AMOS when I co-vary the exogenous constructs, only 2 paths are coming significant out of 6. But when...

03 August 2024 6,028 4 View

Why aren't most metal oxides insoluble in water?

Why BaO (s) + H2O(l) ---> Ba 2+(aq) + 2 OH - (aq) if Oxygen loves owning all the electrons, why would it bind to hydrogen which competes with Oxygen for electron ownership more so than...

03 August 2024 5,392 1 View