I need to show the co-localization of my protein of interest in the membrane in HL60 round and small cells. So I took z-stack images to show the surface expression of the membrane marker that I used. But I don't know how to present my data for the paper.
Frederick Maxfield
We usually present an image of a sum of all the individual planes. We also identfy a point in the image and show the y-z and x-z stacks of the vertical and horizontal planes that go through that point.
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Mahsa Shirin
Dear Frederic,
Thank you for being so helpful. Are you using Fiji for that purpose? or imaris?
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Frederick Maxfield
We usually use a proprietary software called MetaMorph. But either of the two you mentioned should work. I would start with Fiji.
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