Hi..

Generally 9K media is used for Acidothiobacillus ferroxidans. 

Composition of 9K media:

Solution A:

(NH4) 2SO4     3.0 g

KCl                     0.1 g

K2HPO4           0.5 g

MgSO4. 7H2O 0.5 g

Ca(NO3) 2      0.01 g

Agar                 1.5 % (15gm in 1Lt  media)

Distilled water 700.0 ml

Solution B:

FeSO4. 7H2O  44.22 g

H2SO4 (1N)    10.0 ml

Distilled water 290.0 ml

Solution A is autoclaved, Solution B is filter sterilized and then mixed to solution A

asceptically.