How can I prepare suspended solution of fixed WBCs which are stained with wright giemsa ?

26 October 2019 0 6K Report

Hi I try to make sample of stained WBCs for my microfluidics test colored with wright giemsa stain! I want my cells suspended and fixed inside PBS solution !!

Badges
Science topic

More Navid Yarahmadi's questions See All

How two melting peaks in DSC of polypropylene can be merged into one crystallization peak?

The figure shows DSC cooling (blue) and 2nd heating (green) curves (step: 10 K/min) for a commercial sample supplied as Atactic Polypropylene (APP). The heating curve illustrates two major...

03 March 2021 5,857 3 View

Does anyone here uses the Software for Roche LightCycler 2.0 instrument?

Hi everyone, If anyone here uses Roche LightCycler 2.0 instrument for qPCR experiments, I would appreciate if they message me. I have some questions about it. Best - Navid

12 October 2020 6,234 2 View

What is an acceptable FDR% for proteomics data publication?

Is there an established convention on this? I have heard from experts that 1% FDR is recommended. I was wondering can we report protein IDs at 5% FDR. Is there any published guidelines on this? If...

25 August 2020 1,231 4 View

Does the expiration date affect the binding capacity of Ni-NTA?

Hello! I have some expired Ni-NTA resin (QIAGEN), and I need to purify my His-tagged protein. Does the expiration date affect the binding capacity of Ni-NTA? Thank you in advance

11 June 2020 5,773 5 View

How can concrete be modeled with a GFI in ABAQUS?

Hello dears I am modelling concrete in the Abaqus and need to know GFI behavior and parameters them. How can concrete be modeled to the ABAQUS? Thanks

03 March 2020 4,116 3 View

Is there any good free software to simulate electron trajectory in non-uniform electric field?

Need to simulate electron trajectory in non-uniform electric field.

08 February 2020 8,963 3 View

New project in Cathodic Protection?

What fields (topics) do you suggest to work as a metallurgy and Technical Inspection master degree Student in Cathodic Protection? Thank you kindly

17 January 2020 4,201 5 View

Should the effective stresses be constant during the effective stress numerical analysis?

Assuming running the undrained effective stress analysis in FLAC3D via Config FLUID using the effective stiffness and strength parameters, are we expecting the constant effective stresses in the...

15 July 2019 6,096 3 View

How to model the distribution of the population from a facility to other near facilities (for example a school) when it is decided to be destroyed ?

how can we model the distribution of the population from a facility to other near facilities (for example a school or a hospital) when it is decided to be destroyed

25 June 2019 4,220 3 View

Fluorescent staining of bacteria and fungi?

Hi all scientists, I am going to determine the LIVE/ DEAD fungi and bacteria cells with fluorescent dye in the possible biofilm on my plastic films surface. I have TTC (tetrazolium chloride) but...

05 June 2019 2,709 2 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View