Hi I try to make sample of stained WBCs for my microfluidics test colored with wright giemsa stain! I want my cells suspended and fixed inside PBS solution !!
Answer need on urgent base please.
16 January 2024 5,350 5 View
Dear all, I have worked on the gas uptake with MOFs and now I need to calculate IAST selectivity and HOA. I've used python code which is published in some papers but it did not work for me. I...
25 December 2023 5,090 0 View
I'm trying to switch a method established on Agilent Q-TOF to Sciex.
16 August 2023 2,565 2 View
To measure and detect MicroRNAs, researchers face many challenges, Some of them are: 1- Small size 2- Similarity in sequence in the same family 3- A tiny amount in the sample
19 June 2023 6,034 3 View
I have prepared thin films from semi refined carrageenan with hydrophilic cross linkers. But smooth peel off has been the issue for a long time.
06 June 2023 6,014 2 View
I am conducting an earthquake-induced fatigue study on the metal supports of the pressure vessels, which is responsible for the stability of the structure.
11 May 2023 4,649 2 View
At the junction of the metal supports of the pressure tank, a crack has been observed that grows due to the earthquake. How is the fatigue life of the structure confirmed against the lateral force...
14 April 2023 1,296 2 View
determining the fatigue life of metal bases of pressure vessels
14 April 2023 9,456 1 View
I am working on the effect of Markstein number and Markstein length definition in the internal combustion engines. Since mathematically they have a direct relationship, both should have similar...
04 April 2023 7,756 3 View
I have a split split plot design with a main factor(4 levels) and a subfactor (2 levels) and a sub sub factor (2 levels) with 3 replications: 1-How should I define the method on SPSS? 2- for post...
16 January 2023 5,688 8 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...
06 August 2024 1,495 2 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View