How can I prepare carboxyhemoglobin (HbCO)?

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Why I can't see any band in SDS-PAGE?

Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...

06 August 2024 5,373 2 View

Question about water vapor uptake of metal-organic frameworks?

I'm working on the atmospheric water harvesting, and i synthesized MOF by adding some materials into precursor and changing the conditions. (i followed Hydrothermal method) Actually, if MOF is...

24 July 2024 1,515 0 View

Is this a scam?

UK's Well-online Science Press sent an invitation to be a co-author and offers a payment of $30 per review.

22 July 2024 1,695 8 View

When you express a protein, why do we express not only the domain we want, but also the protein around it?

I want to express STK4, and I've searched the paper for reference. When I check the protein kinase domain sequence for that kinase on Uniprot, it's 30-281, but the paper expresses the protein...

20 July 2024 4,951 1 View

The reason for breeding Loxp mice with FLPe mice?

I am planning to use CD4 Cre mice crossed with Loxp mice. However, the laboratory from which I am trying to get the loxp sperm says they have "the product of the EMMA ko crossed with the FLPe to...

16 July 2024 7,475 1 View

Why is serum-free media used when making cancer cell conditioned media?

I am preparing cancer cell conditioned media to study the effects of substances secreted by cancer cells on other cells. In some papers, conditioned media is prepared using FBS-containing media...

11 July 2024 7,063 1 View

Stable cell line generation: GOI is detected by qPCR but protein is not expressed?

Hello, I'm generating stable cell lines by transfecting plasmid DNA with lipofectamine 3000. The cells went through antibiotics selection for 3 weeks (polyclonal selection) and when I analyzed...

11 July 2024 4,812 5 View

NH3/NF3/H2 Remote plasma Oxi/nit selective etching mechanism help ??

Hello, I am an Etch Engineer. I am performing etching with high T_ox/Nit selectivity using remote plasma. Etching plasma is formed using only NH3/NF3/H2. 1. I wonder if it would be advantageous...

11 July 2024 8,115 2 View

Comparison of tissue to serum MS data?

Hello, So I have mouse brain tissue MS data from one of my predecessors and I have human serum data from the hospital that I collaborate with. We are trying to find markers for the diagnosis of...

11 July 2024 8,484 0 View

Is it appropriate to apply the COSMIN guidelines for evaluating the quality of tools used to assess acute postoperative pain?

Hi, I'm a graduate student, college of nursing, chungnam national university in Daejeon. The topic of my thesis is "Quality Assessment of Tools for Evaluating Acute Postoperative Pain." I have...

10 July 2024 3,048 1 View

How to measure accurate concentration of whole protein from RBC?

Although I tried to perform western blotting with protein extract from RBCs, I could not detect the interest of protein due to red background (maybe, heme or hemoglobin ) all over the lane. I...

15 May 2024 6,182 1 View

Outline the principles of airway management in paediatric anaesthesia, including the use of different airway devices and techniques?

The principles of airway management in paediatric anaesthesia are crucial for ensuring adequate oxygenation, ventilation, and prevention of complications during surgery.

09 April 2024 7,753 1 View

Explain the principles of hemodynamic monitoring and its significance in critical care?

Hemodynamic monitoring involves assessing the cardiovascular system's performance to guide the management of critically ill patients. It provides valuable information about cardiac output,...

07 April 2024 1,309 1 View

Na-DL-lactate in a bacterial growth medium - what is the role/function?

Hi! I am prepping a sulfate reducer (Desulfovibrio sp.) medium (M63 from DSMZ https://mediadive.dsmz.de/medium/63) and I am trying to make sense of the formulation. So far my understanding...

12 February 2024 587 4 View

Hello, i need help about the Hemoglobin dissociation curve of fish effected by pH in water enviroment?

When the pH turn low (acidification) in salty-marine water so what change of the Hemoglobin dissociation curve? Is respiratory frequency of a marine fish increase when pH turn low than...

19 January 2024 5,311 1 View

Oxygenation Event during or after the Carboniferous-Permian (type-Karoo) ?

Based on the literature on isotopic compositions of carbon 14 and oxygen 18, each glacial episode is followed by a primordial oxygenation event such as the Great Oxygenation Event (GOE) and the...

04 January 2024 6,571 0 View

How can i deoxygenate oxyhemoglobin solution using nitrogen gas?

I want to deoxygenate oxyhemoglobin with nitrogen bubbling, but i can't observe the characteristic peak of deoxyHb in UV-Vis spectrum. Also, the concentration of my hemoglobin sample was little...

01 January 2024 5,512 0 View

Troubleshooting direct sandwich ELISA?

I am validating an ELISA measuring A1c (also called glycated hemoglobin; target for humans) for use in another species. It is a direct sandwich assay. The standard and my sample (whole blood) were...

25 October 2023 3,471 4 View

I need a protocol to reduce any oxidized (Fe3+) hemoglobin in my sample to (Fe2+)?

Hi everyone, I’m using hemoglobin in the lab and I need it to be in the reduced state (so it is able to bind with CO2). The hemoglobin I have is a lyophilized powder from Sigma Aldrich (H7379)....

13 September 2023 9,133 4 View

Has any previous research been conducted to evaluate the impact of hemolysis, jaundice, and hyperlipidemia on IR spectral profile in serum and plasma?

Only when hemolytic anemia results in a two- to threefold or larger increase in hemoglobin catabolism does jaundice owing to rise of serum unconjugated bilirubin become noticeable. The level of...

07 September 2023 3,103 0 View