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I need to prepare the oxygenated and deoxygenated hemoglobin by bubbling gases such as O2 and N2. In some papers, they started to do experiments with HbCO because it is much stable than the other...
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I prepared deoxyhemoglobin by mixing a few drops of Stokes' reagent into a certain concentration of oxyhemoglobin solution. The deoxyHb was confirmed by UV-vis spectroscopy. I also tried to use...
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