I have a query, I want to do TEM of a Polycaprolactone polymer film (~150-180 micro meter thick). I would like to know what is better wet cryo sectioning or dry cryo sectioning. Can anyone suggest the sample preparation method for its TEM analysis.
Dear Carolina Leimgruber, sample preparation is really a practical skill, may be a specialized staff is needed. Some hint are given RG thread and the attached files. My Regards
https://www.researchgate.net/post/How-can-I-prepare-TEM-samples-of-polymeric-core-shell-nanofibers
Dear Sir,
Microtome cutter widely used for the same. Indeed, it must be handled by specialist as suggested by Prof. Abdelkader .
Please watch this video, it will help you to understand the process and science behind
https://youtu.be/Nl-pE667M0s
Thank you very much, I really appreciate all the data provided.
Maybe, I did not express properly.
I know that I have to cut the sample of Polycaprolactone polymer film at low temperatures using the cryoultramicrotome. I have experience cutting biological samples using the cryoultramicrtome but in the case of the polymer samples I need to know if I can attach the samples to the pin with sucrose for example? I will really appreciate any details about this steps of the technique.
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